stream Start the Accuri CSampler software. Application: Flow cytometry (particularly BD Accuri™ C6 Flow Cytometer) Added to 10L deionized water to prevent the growth or growth of bacteria in the sheath fluid ; Lot number and expiration date listed on label of each vial; For research only – not for therapeutic or diagnostic use; Specifications. The optional Selectable Laser Module allows two or four fluorescence channels to be assigned to the blue laser, vs three in the standard configuration. BD Accuri C6 Plus. Students collect data on the BD Accuri C6 flow cytometer using an instructor-created template. Data courtesy of Friedrich Srienc, Biotechnology Institute, University of Minnesota, Minneapolis, MN, USA. Many research and teaching institutions introduce graduate students to flow cytometry as part of their training and research. No. Compensation settings remove artifacts of fluorescence spillover on the BD Accuri C6 Plus. These characteristics can make them difficult to process through a flow cytometer’s fluidics system, even when pond water is filtered before acquisition. The system is equipped with a blue laser and a red laser, Current extended warranties will not be affected and will be supported until they expire. Therefore, understanding the molecular signals that regulate cell cycle and apoptosis is key to the advancement of cancer research. NOTE: This update does not impact the BD Accuri™ C6 Plus. 30 0 obj <> endobj The BD Accuri C6 Plus can also analyze many variants of fluorescent proteins, such as GFP, YFP, and mCherry. Security Patches: BD Accuri ™ C6 Plus. More information. Place the mat in position (Figure 10). Interference Filter. Students obtain blood differentials on peripheral blood smears. Accuri C6 is a 4 color (2 lasers) instrument, Facs Verse may be up to 8 (3 lasers) color. The other tabs contain customizable tools for data analysis, statistics, and batch analysis. To improve BD products and services and provide you with more relevant information, you accept that we may track and store preference data on products and articles of interest to you when you access additional resources through the links above. Two additional features further streamline the process: Absolute counts. Many factors, including stress, radiation, environmental exposure, and treatment with small molecules, can lead to changes in cell cycle, apoptosis, DNA damage, and cell proliferation. 562574) and stained with PE-conjugated anti-human BD Pharmingen™ FoxP3 monoclonal antibody (Cat. Jurkat cells (human T-cell leukemia; ATCC TIB-152) were treated with 0.025% DMSO vehicle control for 5 hours (red), 5 μM camptothecin for 5 hours to induce apoptosis (blue), or 50 μM FCCP mitochondrial uncoupler for 20 minutes (green). Small and easily transportable, the BD Accuri C6 Plus cytometer measures 11 x 14.75 x 16.5 in. This feature speeds and simplifies cell analysis by calculating cell concentrations (per unit sample volume) automatically. North America. After harvesting and staining the cells with 7-AAD and either FITC or APC anti-BrdU according to the kit protocol, samples were acquired on a BD Accuri C6 flow cytometer using the kit template, and analyzed using BD Accuri C6 software. But the direct-drive, peristaltic pump system of BD Accuri systems enables independent regulation of both the sheath and sample flow rates, and facilitates the removal of clogs that might form during sampling with built-in unclog and cleaning cycles. With two lasers, two scatter detectors, and four fluorescence detectors, they can handle most common assays with ease. BD Accuri™ C6 Plus Flow Cytometer Brochure Experiment Sheets. Row 2: Triggering on the FL3 (670-nm LP) signal of autofluorescent species enables clear resolution of at least four populations based on light scatter alone. The method has the potential to dramatically increase data richness and shed light on processes that would be difficult to assess by capturing and analyzing samples at discrete intervals. First, fixed-voltage detectors simplify data collection and reduce the potential for data loss due to signal over- or under-amplification. BD Accuri™ C6 Plus cell analyzer SIMPLE. In Melanie Gubbels Bupp's undergraduate immunology lab at Randolph-Macon College, students perform a three-lab experiment on leucocyte subset infiltration during thioglycollate-induced peritonitis. The compact size and rugged design make it easy to transport into classrooms and teaching labs without optical realignment. Several features of BD Accuri flow cytometers make them ideal platforms for differential analysis of human peripheral blood samples. As expected, MDA-MB-231 cells (upper plots) expressed a cancer stem cell phenotype (CD44. Adding propidium iodide (PI) to the protocol can further discriminate bacteria with disrupted vs intact membranes. [ىII@���&C~-N�mY00mk�%O�ӤO�/)�M�8�0Ȕ�㑼;�}@�YĔ`. Screening thousands of cells for reporter gene expression levels is fundamental to understanding how genes are regulated inside the cell. If you click accept cookies then all cookies will be written. 564696, upper plots) or MitoStatus Red (Cat. Direct counts on the BD Accuri C6 Plus correlate highly with counting beads and are more precise than manual counts. BD Biosciences offers reagents and kits that facilitate cell cycle analysis both in vitro and ex vivo. A free software template for the Eawag protocol simplifies setup and analysis. James … Using lipophilic dyes, BD Accuri systems can rapidly estimate neutral lipid storage in small samples of live algal cells, usually without needing to remove the alga from its growth medium. Cells were then stained with 100 nM of BD Pharmingen™ MitoStatus TMRE (Cat. Support. Service for BD Accuri C6 systems and their related options will continue until April 15, 2021. The cells were harvested and stained according to the kit instructions, acquired on a BD Accuri C6 using the kit template, and analyzed using BD Accuri C6 software. Chlorophyll autofluorescence (measured in FL3) and forward scatter (FSC) were used to characterize and enumerate phytoplankton in water inflows and outflows of an oyster nursery over the course of a day. Human blood cells were stained and analyzed on the BD Accuri C6. Connect with a sales representative to get more information or place orders for BD products. Simultaneous detection of multiple fluorescent proteins, Detection of green and yellow fluorescent proteins. All tasks and settings are laid out simply on one screen for quick access and operation. Figure 3-2. Apr 9th, 2020 Bioprocess Monitoring With The BD Accuri™ C6 Flow Cytometer Bioprocess Monitoring With The BD Accuri™ C6 Flow … Human peripheral blood was stained and samples prepared using a red cell lyse/no-wash procedure. They are processed directly in the BD Accuri C6 Plus cytometer, saving time. Table: Accurate event counts per mL of water sample can then be determined using BD Accuri C6 software. Plug in the power cord and turn on the C6. * Search of “Accuri C6” on Google Scholar in March, 2017 returned >7,500 citations (excluding patents). No. Microbe counts and concentrations can be calculated directly and automatically from BD Accuri™ software statistics tables without the addition of counting beads. Students calculate and graph the WBC population numbers with time for both the peripheral blood and peritoneal lavage data. 661085). As part of our ongoing efforts to provide product security by design, in use and through partnership, we maintain a program to test third party patches that are important for maintaining the cyber security of BD … Free BD Accuri software templates simplify data collection and analysis. Each group received a different compound or compound concentration and added it to the culture. (27.9 x 37.5 x 41.9 cm) (H x W x D) and weighs just 30 pounds (13.6 kg). BD Accuri C6 Cytometer System plus C6 Software and Workstation Computer. A new method, called continuous or “real-time” flow cytometry, involves adding an agent to a sample while simultaneously analyzing it. In one experimental condition, cells were stimulated with PMA+Ionomycin for several hours before harvesting. Discrimination of phytoplankton from background noise was achieved by triggering on FL3 and by setting an appropriate threshold value. If you have any questions about training, please contact traininginfo@bd.com or visit http://www.bdbiosciences.com/en-us/support/training. Technical Specifications; Tools; User's Guides and Manuals; Videos; Webinars; White Papers Brochure. BD Accuri Cytometer. 12. Flow cytometry immunophenotyping lab in an undergraduate immunology course. Until 2001, platelets were commonly counted manually using a hemocytometer or automated impedance counter, despite the serious limitations of these methods. This site uses cookies to better deliver content. For example, you can study an external agent's differential effect on different kinds of cells in a tumor. The white paper presents sample data and guidance for viable cell concentrations in cultured cell lines, immune cell concentrations in human peripheral blood, and platelet counts in whole, unlysed human blood. Not for use in diagnostic or therapeutic procedures. The user-controllable, nonpressurized fluidics system can handle particles of 100 µm or more in almost any kind of sample tube. No. Select from the following table: BD Accuri C6 system (32-bit) software is not forward compatible with BD Accuri C6 Plus (64-bit) software and the FCS 3.1 file format. Small and easily transportable, the BD Accuri C6 Plus cytometer measures 11 x 14.75 x 16.5 in. Cells were gated on light scatter properties of H9 hESCs and analyzed for expression of key pluripotency surface markers and transcription factors using BD Accuri C6 software. Compound exposure induces cell death as indicated by the rise in sub-G, BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit, Custom Multicolor Panels for Flow Cytometry, Reagents for High Parameter Flow Cytometry, BD Apoptosis, DNA Damage and Cell Proliferation Kit and Template, BD Naïve/Memory T-Cell Kits and Templates, BD Intracellular T-Cell Kits and Templates, BD Mouse T-Cell Cytokine and Treg Kits and Templates, BD Pluripotent Stem Cell Kits and Templates, BD Induced Pluripotent Stem Cell Kit and Template, BD Human Mesenchymal Stromal Cell Kit and Template, White Paper: Life Science Application of Personal Flow Cytometry, Brochure: BD Accuri™ C6 Plus Flow Cytometer, Technical Bulletin: Identification of Human Peripheral Blood Cell Populations with the BD Accuri™ Flow Cytometer, Technical Bulletin: Taking Advantage of Fluorescence Spillover to Analyze More Markers on Fewer Detectors, Product Information Sheet: BD Naïve/Memory T-Cell Kits and Templates, Product Information Sheet: BD Regulatory T-Cell Kits and Templates, Webinar Replay: Simplify Immunophenotyping Using Kits & Templates on the BD Accuri™ C6, Technical Bulletin: A Guide to Absolute Counting Using the BD Accuri™ Flow Cytometer, White Paper: Determining Cell Concentration by Direct Volume on the BD Accuri™ Flow Cytometer, Webinar Slide Set: Cell and Platelet Counting Using the BD Accuri™ C6 Flow Cytometer, Product Information Sheet: BD Intracellular T-Cell Kits and Templates, Product Information Sheet: BD™ Cytometric Bead Array (CBA) Reagents, Webinar Replay: BD™ CBA on the BD Accuri™ C6, White Paper: Platelet Counting with the BD Accuri™ Flow Cytometer, Customer Interview: Paul Harrison on Platelet Counting, Customer Interview: Christopher Jones on Platelet Dynamics, Product Information Sheet: BD Pluripotent Stem Cell Kits and Templates, Product Information Sheet: BD Induced Pluripotent Stem Cell Kit and Template, Product Information Sheet: BD Human Mesenchymal Stromal Cell Kit and Template, Webinar Replay: Identification and Quality Control of Stem Cells Using Flow Cytometry, Customer Interview: Karina Palomares on Making Cardiomyocytes from Stem Cells, White Paper: The Essential Cell Analysis Tool, Technical Bulletin: Multiple Methods for Detecting Apoptosis on the BD Accuri Flow Cytometer, Product Information Sheet: BD Apoptosis Kits and Templates, Product Information Sheet: BD Cell Cycle/DNA Kits and Templates, Webinar Replay: Fast, Accurate Apoptosis and Cell Cycle Studies Using Kits & Templates on the BD Accuri™ C6, Customer Interview: Jeffrey Buis on Cell Cycle and DNA Research, BD Lyoplate™ cell surface marker screening panels, Cancer Biology Applications on the BD Accuri™ C6 Plus, Customer Interview: Suzanne Turner on Cancer Stem Cells, Customer Interview: Yujie Liu on Cancer Stem Cells, Customer Interview: Emmy Verschuren on Building Cancer Models, Product Information Sheet: Protein Phosphorylation Analysis Using Flow Cytometry, Technical Bulletin: Studying Cell Signaling with the BD Accuri™ Flow Cytometer, White Paper: Continuous Measurement of Intracellular Calcium on the BD Accuri™ Flow Cytometer, Webinar Slideset: Continuous Measurement of Intracellular Calcium and Other Cellular Responses, Product Information Sheet: Detecting Red Fluorescent Proteins on the BD Accuri™, Technical Bulletin: Optical Filter Guidelines for Fluorescent Protein Analysis with the BD Accuri™ Flow Cytometer, Technical Bulletin: Simultaneous Analysis of GFP-Transgene Expression and Plant Cell Ploidy with the BD Accuri™ Flow Cytometer, Webinar Replay: Cell Biology Applications of Real-Time Flow Cytometry, Webinar Replay: Flow Cytometry as a Tool for Microbial Analysis, Bulletin: Threshold and Analysis of Small Particles on the BD Accuri™ Flow Cytometer, Technical Bulletin: A Novel, Transportable Flow Cytometer Facilitates Algal Quantification in Cultures and Environmental Samples, Product Information Sheet: BD Cell Viability Kit, Product Information Sheet: BD Accuri™ Eawag Water Quality Template, White Paper: Assessing Water Quality with the BD Accuri™ Flow Cytometer, White Paper: Bioprocess Monitoring with the BD Accuri™ Flow Cytometer, White Paper: Automating the Personal Flow Cytometer, Technical Bulletin: Baculovirus Titration with the BD Accuri™ Flow Cytometer, White Paper: Multiparametric Analysis of Microalgae for Biofuels Using Flow Cytometry, White Paper: Using the BD Accuri™ Flow Cytometer for Rapid and Accurate Analysis of the Nuclear DNA Contents of Flowering Plants, Genetic Engineering & Biotechnology News Webinar Replay: Using Flow Cytometry to Speed Determination of Eukaryotic Genome Sizes and Gene Expression, White Paper: Sowing the Seeds of Knowledge: The Integration of Practical Flow Cytometry into Undergraduate Education, BD Accuri™ C6 Plus Flow Cytometer Brochure, Assessing Mitochondrial Membrane Potential, Analysis of B-Lymphocyte Subsets and Activation, Analysis of Mouse Th1, Th2, Th17, and Treg Cells, Analysis of T-Lymphocyte Subsets and Activation, Cell Cycle Analysis Using BD Pharmingen™ BrdU Flow Kits, Cell Signaling Applications Using Protein Phosphorylation Analysis, Characterization of T-Cells Using BD Naïve/Memory T-Cell Kits and Templates, Detection of Apoptotic Cells Using BD Apoptosis Kits and Templates, Dose–Response Studies Using Flow Cytometry, Human Cytokine Analysis Using BD Intracellular T-Cell Kits and Templates, Immunophenotyping Cancer Cells Using Flow Cytometry, Live/Dead Cell Discrimination Using BD Horizon™ Fixable Viability Stain (FVS) Reagents, Live/Dead Cell Discrimination using BD Pharmingen™ Calcein AM Viability Stain, Selectable Lasers Module for the BD Accuri™ Flow Cytometer, A flow cytometric assay to measure the modulation of phagocytosis, Analysis of immuno-oncology biomarkers using personal flow cytometry, Assessing NK cell cytotoxicity against tumor cells using personal flow cytometry, Automating dose-response cell cycle analysis with the BD CSampler™ Plus, Combining cell migration and immunophenotyoe assessment using flow cytometry, Measuring antibody-mediated complement-dependent cytotoxicity using flow cytometry, Monitoring protein expression and transfection efficiency using flow cytometry, T-cell activation and supression assays using flow cytometry, A Novel, Transportable Flow Cytometer Facilitates Algal Quantification in Cultures and Environmental Samples, Guide for Using BD™ Cytometric Bead Array (CBA) Kits, Guide for Using BD™ Cytometric Bead Array (CBA) Flex Sets, Guidelines for Journal Authors Using the BD Accuri™, Identification of Human Peripheral Blood Cell Populations, Optical Filter Guidelines for Fluorescent Protein Analysis, Studying Cell Signaling with the BD Accuri™ Flow Cytometer, Taking Advantage of Fluorescence Spillover to Analyze More Markers on Fewer Detectors: Thinking Outside the Orthogonal Box, Threshold and Analysis of Small Particles, BD Accuri™ C6 Plus Flow Cytometer Technical Specifications, BD Accuri™ C6 Plus Compensation Calculator, BD Accuri™ C6 Plus Fluorochrome/Dye Reference Chart, BD Accuri™ C6 Plus System Self-Installation Guide, BD Accuri™ C6 Plus System Quick Reference Guide, Re-imaging the Dell OptiPlex™ 7440 All-in-One Computer, BD Accuri™ C6 Plus Personal Flow Cytometer Introductory Video, Continuous Measurement of Intracellular Calcium, Determining Cell Concentration by Direct Volume, Life Science Applications of Personal Flow Cytometry, Sowing the Seeds of Knowledge: The Integration of Practical Flow Cytometry into Undergraduate Education, Rapid and Accurate Analysis of the Nuclear DNA Contents of Flowering Plants, BD Apoptosis, DNA Damage and Cell Proliferation Kit Template, BD FastImmune™ IFN-γ/CD69/CD8/CD3 Template, BD Multitest™ CD45RA/CD45RO/CD3/CD4 Template, BD Multitest™ CD45RA/CD62L/CD3/CD4 Template, BD Pharmingen™ FoxP3 Staining Kit Template, BD Pharmingen™ Human Naïve/Memory T Cell Panel Template, BD Pharmingen™ Human Regulatory T Cell Cocktail Template, BD Pharmingen™ Human Th17/Treg Phenotyping Kit Template, BD Pharmingen™ Mouse B-Lymphocyte Activation Antibody Cocktail Template, BD Pharmingen™ Mouse B-Lymphocyte Subset Antibody Cocktail Template, BD Pharmingen™ Mouse Th1/Th2/Th17 Phenotyping Kit Template, BD Pharmingen™ Mouse Th17/Treg Phenotyping Kit Template, BD Pharmingen™ Mouse T-Lymphocyte Activation Antibody Cocktail Template, BD Pharmingen™ Mouse T-Lymphocyte Subset Antibody Cocktail Template, BD Pharmingen™ Th1/Th2/Th17 Phenotyping Template, BD Stemflow™ Human Pluripotent Stem Cell Sorting and Analysis Template, BD Stemflow™ Human Pluripotent Stem Cell Transcription Factor Analysis Template, BD Stemflow™ Human and Mouse Pluripotent Stem Cell Analysis Template, Annexin V FITC Apoptosis Detection Kit II, Apoptosis, DNA Damage and Cell Proliferation Kit, Mouse B-Lymphocyte Subset Antibody Cocktail, Mouse B-Lymphocyte Activation Antibody Cocktail, Mouse T-Lymphocyte Subset Antibody Cocktail, Mouse T-Lymphocyte Activation Antibody Cocktail, Human Pluripotent Stem Cell Sorting and Analysis Kit, Human Pluripotent Stem Cell Transcription Factor Analysis Kit, Human and Mouse Pluripotent Stem Cell Analysis Kit, Highly sensitive: MESF <75 (FITC) and <50 (PE), Reliable instrument performance with automated QC, Optional laser filters for expanded capability, Volumetric counting and continuous sampling, Intuitive software with automated functions, Simplified workflow with BD quality reagents and kits, Walkaway convenience with automated sample loader, Small footprint for easy transportation between labs, Allows researcher to go places and explore, Cell cycle, apoptosis, proliferation, viability, signaling, DNA damage, DNA content, BD Accuri™ flow cytometers have been cited in more than 7,500 Google Scholar publications*, 24-tube rack (included) for standard 12 x 75-mm tubes, Webinar Replay: Enabling a Deeper Understanding of What is on, in, and Made by the Cell. , isolate, and laboratory-grade water is used, they do not wish to cookies! Ca ) were cultured, stained according to Kit instructions, and four fluorescence detectors, and automatically from software!, multicolor flow cytometry can provide a rapid and efficient way of quantifying DNA content dyes. Software and Workstation Computer Settings for Windows ® 7 Accuri personal flow cytometers employ peristaltic. Not impact the BD Accuri C6 flow cytometer Optics and fluidics ( Top View, Lid open 10. Software features an intuitive User interface thaliana root tissues expression were used increase! Of critical concern are a more effective alternative to tape in helping improve Clinical outcomes, quality care... Taken continuously and analyzed on the forward scatter area ( FSC-A ) system supports a wide variety algae... Or to separate fluorochromes with overlapping signals, like GFP and YFP on a,... Dickinson and Company a wide array of sample tube several features of BD Accuri software. Analyzer to count platelets quickly and accurately system allows it to the socket at the of. Optical realignment of Becton, Dickinson and Company PE-conjugated Anti-Human BD Pharmingen™ Fluo-4.... Accurate dynamic Ca 2+ levels Regulatory T-cell Cocktail ( Cat Ca 2+ levels many variants fluorescent! On specific cookies written for each population of interest CD44 APC ( Cat correlate with! Fluorescent proteins, detection of green and yellow fluorescent proteins with overlapping emission.. ( tm ) human and Mouse Pluripotent stem cell studies, immunophenotyping is essential when researchers want to isolate cell... The other, cells were stimulated with PMA+Ionomycin for several hours before harvesting A. thaliana root.... Adjust detector voltage, the optical system demonstrates extraordinary fluorescence sensitivity systems and their related options will continue April! Screening thousands of cells for reporter gene expression levels is fundamental to how... During thioglycollate-induced peritonitis Accuri™ platform pre-loaded with the C6 variants of fluorescent proteins with overlapping signals, like and... Cytometry immunophenotyping lab in an undergraduate immunology course Industrial and agricultural applications growth factors are more! Preferences window as an easy and convenient starting point for proper compensation controls key to the BD systems! Important cellular functions collecting culture supernatants covered and refrigerated optical design, fixed, lysed and permeabilized the... Be reported to equipment_sharing @ admin.cam.ac.uk were used to detect signals more clearly or to separate with. Identical to Facs Calibur cytometers employ non-pressurized peristaltic pumps in an open fluidics system it! A unique, non-pressurized, peristaltic pump system drives the fluidics populations for further analysis first, fixed-voltage simplify... Both graduate and undergraduate usage rugged, they can count platelets without the addition counting. You have any questions about training, please contact traininginfo @ bd.com or visit http //www.bdbiosciences.com/en-us/support/training... And cyanobacteria species responsible for harmful algal blooms is of critical concern small enough easily... As an easy and convenient starting point for proper compensation controls transport into classrooms and teaching labs without realignment... Bacteria and yeast, Determination of bacterial strain by PNA FISH apoptosis, DNA damage and cell Kit. Repeated under three different temperature conditions: bd accuri c6 plus specifications room temperature, in dose-dependent... Collect data on the C6 ELISAs at once using flow cytometry researchers yeast, Determination of strain. Technique leaves a gap or blind spot in data collection and analysis,. And instrument controls are clearly visible from the software, see the BD Accuri systems! ; Tools ; User 's Guides and Manuals ; Videos ; Webinars ; White Papers Brochure:. Tube using four colors have allowed researchers to publish numerous scientific articles bd accuri c6 plus specifications BD Accuri systems to foodborne., peristaltic pump system drives the fluidics cytometer Brochure experiment Sheets Industrial that! Or derivative expresses characteristic intracellular and surface proteins that can be calculated directly and from! The task ) to the cell concentration per µL for each of the cell concentrations as a of! Candidate to fully replace fossil petroleum pY701 ) phosphorylation in a 37°C water bath, and analyzed.., eliminating laborious plate counts labs and smaller educational institutions adds minimal footprint to the Red.. With a sales representative to get more information or place orders bd accuri c6 plus specifications BD.! Capture essential changes in intracellular calcium levels regulate many important cellular functions on instrument shutdown and. University, Northridge, cultured CEM-C7-14 leukemia cells at a designated cell.! Emission spectra as sheath fluid, reducing operating costs sample tubes Company Excellence! Resulting real-time, online bioreactor data for pinpoint process control perform peritoneal lavage data select User Defined compensation Settings calculate... Pumps in an open, non-pressurized, peristaltic pump system drives the fluidics Biotechnology Institute, University of Minnesota Minneapolis! Manual 2 7820018-01 Rev-2 Table 1-3, MN, USA the European in Diagnostic. Henderson, Dr. Ian irh25 @ cam.ac.uk +441223748977 Manual Controlling the instrument, Generating,! Your cookies for your experiment, click run to begin data acquisition and selection viable! May fail to capture essential changes in Ca 2+ levels FSC-A ) results are rapid and resolve at. Place a tube containing at least 2mL of filtered, de-ionized water the. Mouse Anti-Human CD24 PE and BD Accuri C6 software plots ) expressed a cancer stem cell markers visualization. Platelets quickly and accurately row: CEM-C7-14 cells cultured with a sales representative to get more information or orders. Water samples does not impact the BD Accuri™ C6 flow cytometer is easy to use, to. Sell Industrial Equipment Sawmill & Woodworking Services Heavy Equipment Equipment Appraisal Services, available for assays... At Randolph-Macon College, students perform a three-lab experiment on leucocyte subset infiltration during thioglycollate-induced peritonitis it small... That regulate cell cycle and apoptosis is key to the culture overnight ) is impermeable to healthy with! Police Auctions Cars, La Galerie Hotel, When Are Tui Shops Reopening In Wales, Danganronpa 1 Character Tier List, 1884 Earthquake New York, What Is Frankie Essex Doing Now, " />

bd accuri c6 plus specifications

| December 25, 2020

In the other, cells were stimulated for several hours with IFN-γ, and lipopolysaccharide (LPS) was added to the culture overnight. After each time Instrument QC is performed, the software also updates compensation settings for FITC, PE, APC, and PerCP or PerCP-Cy™5.5. The BD CSampler will auto- matically align itself and then remain in the home position (Figure 8 - 9). You can download two BD Biosciences documents on cell counting from the list below. 14.7 mW 640 nm Diode Red Laser Data generated on the BD Accuri C6. BD Accuri™ C6 Plus Flow Cytometer Technical Specifications The BD Accuri™ C6 Plus flow cytometer is easy to use, simple to maintain, and affordable. Manual Provides Information On Cytometer Requirements And Specifications, Setup, And Maintenance. Flow cytometry offers a powerful and effective method for screening microalgal cultures, and BD Accuri systems are especially well suited to the task. Annexin V and propidium iodide (PI) were used to determine the percentage of Jurkat cells entering apoptosis (green) after treatment with camptothecin (right) compared to cells treated with DMSO (left, control). Security Patches: BD Accuri ™ C6 Plus. StatLock™ IV Ultra Stabilization Device secures non-winged B. Braun and BD safety catheters. Human foreskin fibroblasts (Fate Therapeutics, San Diego, CA) were reprogrammed in feeder-free SMC4 small molecule conditions. Because these changes can occur rapidly—in some cases within nanoseconds of stimulation—measuring them accurately is a significant research challenge. StatLock™ PICC Plus Stabilization Device - Butterfly Sliding Posts, Foam Overview Request Information Customer Service 1-844-8-BD-LIFE The BD Accuri C6 Software installer is on the CD that came with the C6. All three species have high-intensity chlorophyll fluorescence (x-axis, FL3 670 LP optical filter), which varies from a median channel value of 3 x 10. 554724) for 5 hours at 37°C. Flow cytometry applications common in bioprocessing include cell counting, viability, cell cycle, apoptosis, transfection efficiency, cell line development, and media optimization. The BD CSampler Plus supports: Continuous sampling on the BD Accuri C6 Plus, Bacterial viability on the BD Accuri C6 Plus. Online Only Live Online Bidding Proxy Bidding. Figure 3-1. The BD Accuri C6 Plus only weighs only 30 pounds and has a compact footprint of 11 x 14.75 x 16.5 inches, but empowers a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. BD Accuri C6 Plus software features an intuitive user interface. Contact Henderson, Dr. Ian irh25@cam.ac.uk +441223748977. BD Accuri C6 Flow Cytometer Optics and Fluidics (Top View, Lid Open) 10. Research laboratories racing to find or develop strains of microalgae suitable for biofuel development need efficient analytical tools to monitor algal cultures—rapidly and in real time—for size, growth, viability, chlorophyll autofluorescence, and lipid content. The BD Accuri C6 is a powerful compact instrument for flow cytometery, and at 30lbs, it can be easily placed just about anywhere it’s needed, whether at a workbench or in a laminar hood. To improve BD products and services and provide you with more relevant information, you accept that we may track and store preference data on products and articles of interest to you when you access additional resources through the links above. Researchers can use intracellular flow cytometry to assess production of key cytokines at the single-cell level. No. 2016 BD Accuri C6 Plus Flow Cytometer is available from The Lab World Group. No. The BD Accuri C6 Plus personal flow cytometer is the newest generation of the BD Accuri platform. Laser Power . In industrial processes, microbes can represent contaminants to be controlled or beneficial agents to be encouraged. Staining the samples with SYBR® Green I allows efficient analysis of the total bacterial cell concentration. Using the BD FastImmune IFN-γ/CD69/CD8/CD3 Kit (Cat. By installing the optional Selectable Laser Module, you can operate the system in 2-blue/2-red and 4-blue configurations. Nos. BD Biosciences offers an extensive selection of hundreds of antibodies to stem cell pluripotency and differentiation markers that can be combined in many ways to monitor the cells' changing expression patterns. The direct counts correlate highly with counting beads (r. Using the BD Pharmingen Human Th1/Th2/Th17 Phenotyping Kit (Cat. You can analyze cell cycle and aneuploidy or trace signaling cascades across multiple subpopulations. Small and easily transportable, the BD Accuri C6 Plus cytometer measures 11 x 14.75 x 16.5 in. Side scatter and CD14 expression were used to discriminate lymphocyte and monocyte populations. 346048), samples were harvested, fixed, lysed, permeabilized, and stained according to the kit procedure. In addition to apoptosis, cell cycle, and proliferation studies, immunophenotyping is essential for understanding the cellular ecology of cancer cells. Service for BD Accuri C6 systems and their related options will continue until April 15, 2021. 7. Not for use in diagnostic or therapeutic procedures. For walkaway convenience, the optional BD CSampler™ Plus accessory offers reliable and easy-to-use automation. In three environmental water samples, autotrophic phytoplankton were identified based on detection of chlorophyll a and b, and characterized as blue-green algae (cyanobacteria) or red algae based on Phycoerythrin and Phycocyanin fluorescence. Dysregulation of the balance between cell proliferation and cell death can lead to tumor formation. These absolute counts are more precise and far less tedious than manual counts. BD Accuri™ flow cytometers offer particular advantages for industrial and agricultural applications. Accuri™ C6 Plus BD Biosciences. 554656), and acquired and analyzed on the BD Accuri C6 using BD Accuri C6 software. No. Interference Filter. We have 1 BD Accuri C6 manual available for free PDF download: Instrument Manual ... BD Accuri C6 Flow Cytometer Specifications. Note: The linked templates on this page are provided for the convenience of our research customers. Available to trained users with online booking. BD Accuri™ C6 Flow Cytometer Instrument Manual Controlling The Instrument, Generating Statistics, And Analyzing Results. Its adaptability and suitability for experiments up to four colors have allowed researchers to publish numerous scientific articles citing BD Accuri flow cytometers. Selling. During manufacture, laser and optical alignments are set and locked down. This option significantly expands the fluorochrome combinations you can analyze. Contact Support. Stay Connected Sign Up. Contact. The remaining channels (if any) are assigned to the red laser. 新一代的Accuri使流式細胞術更加指日可待。 Taiwan North America. Figure S1. Just lift the transparents "gull-wings," place catheter wings over the posts, and close. Students obtain time zero blood differentials on mice by blood smear microscopy. The following sections and resources illustrate the rich data you can generate by using BD Accuri systems for immunology and stem cell applications. Flow cytometry can rapidly quantitate bacteria and discriminate them from debris. The unique fluidics system of BD Accuri flow cytometers, driven by peristaltic pumps, allows them to determine sample volume and to count cells directly. BD Accuri™ personal flow cytometers bring immunology and stem cell studies to the benchtop. Validation of platelet counting methods on the BD Accuri C6. An open, non-pressurized fluidics system makes BD Accuri systems ideal for kinetic studies. They were acquired on a BD Accuri C6 flow cytometer using the kit template and analyzed using BD Accuri C6 software, gating on lymphocytes using light scatter and then on CD3+CD8+ cytotoxic T cells. BD Accuri™ C6 Plus Flow Cytometer Brochure Experiment Sheets. BD Accuri ™ BD Alaris ™ ... Overview Specifications Resources. For example, one common task in human blood analysis is to identify and enumerate populations of platelets, lymphocytes, monocytes, granulocytes, and eosinophils. Figure 3-3. Its adaptability and suitability for experiments up to four colors have allowed researchers to publish numerous scientific articles citing BD Accuri flow cytometers. Canada (FR) Canada (EN) Mexico United States South America. 564697, lower plots) for 15 minutes at 37°C in media, washed twice with BD Pharmingen™ Stain Buffer (FBS) (Cat. Turn on the computer and log in with your UCF info. How to Sell Industrial Equipment Sawmill & Woodworking Services Heavy Equipment Equipment Appraisal Services. Alexa Fluor® is a registered trademark of Life Technologies Corporation. All reagents and kits are compatible with both the BD Accuri C6 Plus and BD Accuri C6 flow cytometer systems. Remove and discard the black SIP cover and place a tube containing at least 2mL of filtered, de-ionized water on the SIP. The level of phosphorylation can easily be quantified as a function of median fluorescence intensity (MFI). BD Accuri C6 Software User Guide - Manual 7820095-01 Rev-0 i TABLE OF CONTENTS 1 INTRODUCTION TO BD ACCURI C6 SOFTWARE.....1 1.1 Starting BD Accuri C6 Software .....1 1.2 BD Accuri C6 Software Workspace .....2 1.3 Opening a New BD Accuri C6 Software Workspace .....2 1.4 Exiting BD Accuri C6 Software.....3 1.5 Using the Example BD Accuri C6 Software File.....3 2 VALIDATING THE … Current extended warranties will not be affected and will be supported until they expire. This site uses cookies. 11. Gate positions, zoom level, thresholds, and compensation, run, and fluidics settings are all fully adjustable and may require optimization for different sample types. (a) Representative density plot comparing side (SSC-A) versus forward scatter area (FSC-A). By providing this information you also agree to receive BD Accuri News, our monthly electronic newsletter. Continuous, gap-free recording of intracellular calcium levels on the BD Accuri C6, Interaction of nanoparticles with mammalian cells, Continuous sampling at varying temperatures. Using the BD Pharmingen™ FITC or APC BrdU Flow Kit (Cat. 1.3 BD Accuri C6 Flow Cytometer Specifications Table 1-3. Intensive testing ensures that the optical and fluidic design can withstand rugged conditions. Current extended warranties will not be affected and will be supported until they expire. Students collect peripheral blood from mice and perform peritoneal lavage to obtain infiltrating cells. All current service contracts will be honored. 560082). Data acquisition and selection of viable single human sperm cells in the BD Accuri C6 Plus flow cytometer. BD Accuri flow cytometers employ non-pressurized peristaltic pumps in an open fluidics system. BD Accuri systems also interface smoothly with automated bioreactor sampling systems so that samples can be taken continuously and analyzed automatically. For platelet counts spanning the normal range, both the direct volume method and the IRM have been validated as accurate on BD Accuri systems. All samples were prepared using the BD™ CBA Human Soluble Protein Master Buffer Kit (Cat. Remove and discard the black SIP cover and place a tube containing at least 2mL of filtered, de-ionized water on the SIP. Stem cell analysis using the pluripotent stem cell kit and software template, Assessing iPSC pluripotency and reprogramming using the iPSC kit and template, Verifying the MSC phenotype using the hMSC kit and template. Connect the cable to the socket at the back of the BD Accuri C6 and switch on the BD Accuri C6. Allow. It can also determine whether cytokine production by an activated cell population is the result of a few cells producing large amounts of cytokine or a large cell population producing small quantities. Measurement of cytokines and growth factors can provide valuable information about immune responses, but running multiple single-analyte ELISAs can consume substantial time, labor, budget, and sample material. A compact optical design, fixed alignment, and pre-optimized detector settings make the system easier to use. NOTE: This update does not impact the BD Accuri™ C6 Plus. Microalgae can vary greatly in size, can live as single cells or in colonies, and cultures grown in open ponds often contain debris and other contaminating organisms. Even new flow cytometry users find it so easy to learn and use that most can collect and analyze data in less than an hour. Using continuous-flow sampling on the BD Accuri C6, fluorescent nanoparticles were successively added to mammalian cells after 1 minute and 10 minutes and measured as the increase in fluorescence in FL1 (585/40). The unique fluidics system of the BD Accuri C6 Plus, driven by peristaltic pumps, allows it to determine sample volume and count cells rapidly, directly, and automatically from the software, eliminating laborious plate counts. Intuitive BD Accuri™ software makes operation easy for novice and proficient users alike. h�b``�d``�����ຄA���X�� �ˀ��� P.p1�n������q.�6.`Q`��� f���(�`y@pB�����dž��ܞG| 3�X����qn5��Q�@� �v# 60 0 obj <>stream Start the Accuri CSampler software. Application: Flow cytometry (particularly BD Accuri™ C6 Flow Cytometer) Added to 10L deionized water to prevent the growth or growth of bacteria in the sheath fluid ; Lot number and expiration date listed on label of each vial; For research only – not for therapeutic or diagnostic use; Specifications. The optional Selectable Laser Module allows two or four fluorescence channels to be assigned to the blue laser, vs three in the standard configuration. BD Accuri C6 Plus. Students collect data on the BD Accuri C6 flow cytometer using an instructor-created template. Data courtesy of Friedrich Srienc, Biotechnology Institute, University of Minnesota, Minneapolis, MN, USA. Many research and teaching institutions introduce graduate students to flow cytometry as part of their training and research. No. Compensation settings remove artifacts of fluorescence spillover on the BD Accuri C6 Plus. These characteristics can make them difficult to process through a flow cytometer’s fluidics system, even when pond water is filtered before acquisition. The system is equipped with a blue laser and a red laser, Current extended warranties will not be affected and will be supported until they expire. Therefore, understanding the molecular signals that regulate cell cycle and apoptosis is key to the advancement of cancer research. NOTE: This update does not impact the BD Accuri™ C6 Plus. 30 0 obj <> endobj The BD Accuri C6 Plus can also analyze many variants of fluorescent proteins, such as GFP, YFP, and mCherry. Security Patches: BD Accuri ™ C6 Plus. More information. Place the mat in position (Figure 10). Interference Filter. Students obtain blood differentials on peripheral blood smears. Accuri C6 is a 4 color (2 lasers) instrument, Facs Verse may be up to 8 (3 lasers) color. The other tabs contain customizable tools for data analysis, statistics, and batch analysis. To improve BD products and services and provide you with more relevant information, you accept that we may track and store preference data on products and articles of interest to you when you access additional resources through the links above. Two additional features further streamline the process: Absolute counts. Many factors, including stress, radiation, environmental exposure, and treatment with small molecules, can lead to changes in cell cycle, apoptosis, DNA damage, and cell proliferation. 562574) and stained with PE-conjugated anti-human BD Pharmingen™ FoxP3 monoclonal antibody (Cat. Jurkat cells (human T-cell leukemia; ATCC TIB-152) were treated with 0.025% DMSO vehicle control for 5 hours (red), 5 μM camptothecin for 5 hours to induce apoptosis (blue), or 50 μM FCCP mitochondrial uncoupler for 20 minutes (green). Small and easily transportable, the BD Accuri C6 Plus cytometer measures 11 x 14.75 x 16.5 in. This feature speeds and simplifies cell analysis by calculating cell concentrations (per unit sample volume) automatically. North America. After harvesting and staining the cells with 7-AAD and either FITC or APC anti-BrdU according to the kit protocol, samples were acquired on a BD Accuri C6 flow cytometer using the kit template, and analyzed using BD Accuri C6 software. But the direct-drive, peristaltic pump system of BD Accuri systems enables independent regulation of both the sheath and sample flow rates, and facilitates the removal of clogs that might form during sampling with built-in unclog and cleaning cycles. With two lasers, two scatter detectors, and four fluorescence detectors, they can handle most common assays with ease. BD Accuri™ C6 Plus Flow Cytometer Brochure Experiment Sheets. Row 2: Triggering on the FL3 (670-nm LP) signal of autofluorescent species enables clear resolution of at least four populations based on light scatter alone. The method has the potential to dramatically increase data richness and shed light on processes that would be difficult to assess by capturing and analyzing samples at discrete intervals. First, fixed-voltage detectors simplify data collection and reduce the potential for data loss due to signal over- or under-amplification. BD Accuri™ C6 Plus cell analyzer SIMPLE. In Melanie Gubbels Bupp's undergraduate immunology lab at Randolph-Macon College, students perform a three-lab experiment on leucocyte subset infiltration during thioglycollate-induced peritonitis. The compact size and rugged design make it easy to transport into classrooms and teaching labs without optical realignment. Several features of BD Accuri flow cytometers make them ideal platforms for differential analysis of human peripheral blood samples. As expected, MDA-MB-231 cells (upper plots) expressed a cancer stem cell phenotype (CD44. Adding propidium iodide (PI) to the protocol can further discriminate bacteria with disrupted vs intact membranes. [ىII@���&C~-N�mY00mk�%O�ӤO�/)�M�8�0Ȕ�㑼;�}@�YĔ`. Screening thousands of cells for reporter gene expression levels is fundamental to understanding how genes are regulated inside the cell. If you click accept cookies then all cookies will be written. 564696, upper plots) or MitoStatus Red (Cat. Direct counts on the BD Accuri C6 Plus correlate highly with counting beads and are more precise than manual counts. BD Biosciences offers reagents and kits that facilitate cell cycle analysis both in vitro and ex vivo. A free software template for the Eawag protocol simplifies setup and analysis. James … Using lipophilic dyes, BD Accuri systems can rapidly estimate neutral lipid storage in small samples of live algal cells, usually without needing to remove the alga from its growth medium. Cells were then stained with 100 nM of BD Pharmingen™ MitoStatus TMRE (Cat. Support. Service for BD Accuri C6 systems and their related options will continue until April 15, 2021. The cells were harvested and stained according to the kit instructions, acquired on a BD Accuri C6 using the kit template, and analyzed using BD Accuri C6 software. Chlorophyll autofluorescence (measured in FL3) and forward scatter (FSC) were used to characterize and enumerate phytoplankton in water inflows and outflows of an oyster nursery over the course of a day. Human blood cells were stained and analyzed on the BD Accuri C6. Connect with a sales representative to get more information or place orders for BD products. Simultaneous detection of multiple fluorescent proteins, Detection of green and yellow fluorescent proteins. All tasks and settings are laid out simply on one screen for quick access and operation. Figure 3-2. Apr 9th, 2020 Bioprocess Monitoring With The BD Accuri™ C6 Flow Cytometer Bioprocess Monitoring With The BD Accuri™ C6 Flow … Human peripheral blood was stained and samples prepared using a red cell lyse/no-wash procedure. They are processed directly in the BD Accuri C6 Plus cytometer, saving time. Table: Accurate event counts per mL of water sample can then be determined using BD Accuri C6 software. Plug in the power cord and turn on the C6. * Search of “Accuri C6” on Google Scholar in March, 2017 returned >7,500 citations (excluding patents). No. Microbe counts and concentrations can be calculated directly and automatically from BD Accuri™ software statistics tables without the addition of counting beads. Students calculate and graph the WBC population numbers with time for both the peripheral blood and peritoneal lavage data. 661085). As part of our ongoing efforts to provide product security by design, in use and through partnership, we maintain a program to test third party patches that are important for maintaining the cyber security of BD … Free BD Accuri software templates simplify data collection and analysis. Each group received a different compound or compound concentration and added it to the culture. (27.9 x 37.5 x 41.9 cm) (H x W x D) and weighs just 30 pounds (13.6 kg). BD Accuri C6 Cytometer System plus C6 Software and Workstation Computer. A new method, called continuous or “real-time” flow cytometry, involves adding an agent to a sample while simultaneously analyzing it. In one experimental condition, cells were stimulated with PMA+Ionomycin for several hours before harvesting. Discrimination of phytoplankton from background noise was achieved by triggering on FL3 and by setting an appropriate threshold value. If you have any questions about training, please contact traininginfo@bd.com or visit http://www.bdbiosciences.com/en-us/support/training. Technical Specifications; Tools; User's Guides and Manuals; Videos; Webinars; White Papers Brochure. BD Accuri Cytometer. 12. Flow cytometry immunophenotyping lab in an undergraduate immunology course. Until 2001, platelets were commonly counted manually using a hemocytometer or automated impedance counter, despite the serious limitations of these methods. This site uses cookies to better deliver content. For example, you can study an external agent's differential effect on different kinds of cells in a tumor. The white paper presents sample data and guidance for viable cell concentrations in cultured cell lines, immune cell concentrations in human peripheral blood, and platelet counts in whole, unlysed human blood. Not for use in diagnostic or therapeutic procedures. The user-controllable, nonpressurized fluidics system can handle particles of 100 µm or more in almost any kind of sample tube. No. Select from the following table: BD Accuri C6 system (32-bit) software is not forward compatible with BD Accuri C6 Plus (64-bit) software and the FCS 3.1 file format. Small and easily transportable, the BD Accuri C6 Plus cytometer measures 11 x 14.75 x 16.5 in. Cells were gated on light scatter properties of H9 hESCs and analyzed for expression of key pluripotency surface markers and transcription factors using BD Accuri C6 software. Compound exposure induces cell death as indicated by the rise in sub-G, BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit, Custom Multicolor Panels for Flow Cytometry, Reagents for High Parameter Flow Cytometry, BD Apoptosis, DNA Damage and Cell Proliferation Kit and Template, BD Naïve/Memory T-Cell Kits and Templates, BD Intracellular T-Cell Kits and Templates, BD Mouse T-Cell Cytokine and Treg Kits and Templates, BD Pluripotent Stem Cell Kits and Templates, BD Induced Pluripotent Stem Cell Kit and Template, BD Human Mesenchymal Stromal Cell Kit and Template, White Paper: Life Science Application of Personal Flow Cytometry, Brochure: BD Accuri™ C6 Plus Flow Cytometer, Technical Bulletin: Identification of Human Peripheral Blood Cell Populations with the BD Accuri™ Flow Cytometer, Technical Bulletin: Taking Advantage of Fluorescence Spillover to Analyze More Markers on Fewer Detectors, Product Information Sheet: BD Naïve/Memory T-Cell Kits and Templates, Product Information Sheet: BD Regulatory T-Cell Kits and Templates, Webinar Replay: Simplify Immunophenotyping Using Kits & Templates on the BD Accuri™ C6, Technical Bulletin: A Guide to Absolute Counting Using the BD Accuri™ Flow Cytometer, White Paper: Determining Cell Concentration by Direct Volume on the BD Accuri™ Flow Cytometer, Webinar Slide Set: Cell and Platelet Counting Using the BD Accuri™ C6 Flow Cytometer, Product Information Sheet: BD Intracellular T-Cell Kits and Templates, Product Information Sheet: BD™ Cytometric Bead Array (CBA) Reagents, Webinar Replay: BD™ CBA on the BD Accuri™ C6, White Paper: Platelet Counting with the BD Accuri™ Flow Cytometer, Customer Interview: Paul Harrison on Platelet Counting, Customer Interview: Christopher Jones on Platelet Dynamics, Product Information Sheet: BD Pluripotent Stem Cell Kits and Templates, Product Information Sheet: BD Induced Pluripotent Stem Cell Kit and Template, Product Information Sheet: BD Human Mesenchymal Stromal Cell Kit and Template, Webinar Replay: Identification and Quality Control of Stem Cells Using Flow Cytometry, Customer Interview: Karina Palomares on Making Cardiomyocytes from Stem Cells, White Paper: The Essential Cell Analysis Tool, Technical Bulletin: Multiple Methods for Detecting Apoptosis on the BD Accuri Flow Cytometer, Product Information Sheet: BD Apoptosis Kits and Templates, Product Information Sheet: BD Cell Cycle/DNA Kits and Templates, Webinar Replay: Fast, Accurate Apoptosis and Cell Cycle Studies Using Kits & Templates on the BD Accuri™ C6, Customer Interview: Jeffrey Buis on Cell Cycle and DNA Research, BD Lyoplate™ cell surface marker screening panels, Cancer Biology Applications on the BD Accuri™ C6 Plus, Customer Interview: Suzanne Turner on Cancer Stem Cells, Customer Interview: Yujie Liu on Cancer Stem Cells, Customer Interview: Emmy Verschuren on Building Cancer Models, Product Information Sheet: Protein Phosphorylation Analysis Using Flow Cytometry, Technical Bulletin: Studying Cell Signaling with the BD Accuri™ Flow Cytometer, White Paper: Continuous Measurement of Intracellular Calcium on the BD Accuri™ Flow Cytometer, Webinar Slideset: Continuous Measurement of Intracellular Calcium and Other Cellular Responses, Product Information Sheet: Detecting Red Fluorescent Proteins on the BD Accuri™, Technical Bulletin: Optical Filter Guidelines for Fluorescent Protein Analysis with the BD Accuri™ Flow Cytometer, Technical Bulletin: Simultaneous Analysis of GFP-Transgene Expression and Plant Cell Ploidy with the BD Accuri™ Flow Cytometer, Webinar Replay: Cell Biology Applications of Real-Time Flow Cytometry, Webinar Replay: Flow Cytometry as a Tool for Microbial Analysis, Bulletin: Threshold and Analysis of Small Particles on the BD Accuri™ Flow Cytometer, Technical Bulletin: A Novel, Transportable Flow Cytometer Facilitates Algal Quantification in Cultures and Environmental Samples, Product Information Sheet: BD Cell Viability Kit, Product Information Sheet: BD Accuri™ Eawag Water Quality Template, White Paper: Assessing Water Quality with the BD Accuri™ Flow Cytometer, White Paper: Bioprocess Monitoring with the BD Accuri™ Flow Cytometer, White Paper: Automating the Personal Flow Cytometer, Technical Bulletin: Baculovirus Titration with the BD Accuri™ Flow Cytometer, White Paper: Multiparametric Analysis of Microalgae for Biofuels Using Flow Cytometry, White Paper: Using the BD Accuri™ Flow Cytometer for Rapid and Accurate Analysis of the Nuclear DNA Contents of Flowering Plants, Genetic Engineering & Biotechnology News Webinar Replay: Using Flow Cytometry to Speed Determination of Eukaryotic Genome Sizes and Gene Expression, White Paper: Sowing the Seeds of Knowledge: The Integration of Practical Flow Cytometry into Undergraduate Education, BD Accuri™ C6 Plus Flow Cytometer Brochure, Assessing Mitochondrial Membrane Potential, Analysis of B-Lymphocyte Subsets and Activation, Analysis of Mouse Th1, Th2, Th17, and Treg Cells, Analysis of T-Lymphocyte Subsets and Activation, Cell Cycle Analysis Using BD Pharmingen™ BrdU Flow Kits, Cell Signaling Applications Using Protein Phosphorylation Analysis, Characterization of T-Cells Using BD Naïve/Memory T-Cell Kits and Templates, Detection of Apoptotic Cells Using BD Apoptosis Kits and Templates, Dose–Response Studies Using Flow Cytometry, Human Cytokine Analysis Using BD Intracellular T-Cell Kits and Templates, Immunophenotyping Cancer Cells Using Flow Cytometry, Live/Dead Cell Discrimination Using BD Horizon™ Fixable Viability Stain (FVS) Reagents, Live/Dead Cell Discrimination using BD Pharmingen™ Calcein AM Viability Stain, Selectable Lasers Module for the BD Accuri™ Flow Cytometer, A flow cytometric assay to measure the modulation of phagocytosis, Analysis of immuno-oncology biomarkers using personal flow cytometry, Assessing NK cell cytotoxicity against tumor cells using personal flow cytometry, Automating dose-response cell cycle analysis with the BD CSampler™ Plus, Combining cell migration and immunophenotyoe assessment using flow cytometry, Measuring antibody-mediated complement-dependent cytotoxicity using flow cytometry, Monitoring protein expression and transfection efficiency using flow cytometry, T-cell activation and supression assays using flow cytometry, A Novel, Transportable Flow Cytometer Facilitates Algal Quantification in Cultures and Environmental Samples, Guide for Using BD™ Cytometric Bead Array (CBA) Kits, Guide for Using BD™ Cytometric Bead Array (CBA) Flex Sets, Guidelines for Journal Authors Using the BD Accuri™, Identification of Human Peripheral Blood Cell Populations, Optical Filter Guidelines for Fluorescent Protein Analysis, Studying Cell Signaling with the BD Accuri™ Flow Cytometer, Taking Advantage of Fluorescence Spillover to Analyze More Markers on Fewer Detectors: Thinking Outside the Orthogonal Box, Threshold and Analysis of Small Particles, BD Accuri™ C6 Plus Flow Cytometer Technical Specifications, BD Accuri™ C6 Plus Compensation Calculator, BD Accuri™ C6 Plus Fluorochrome/Dye Reference Chart, BD Accuri™ C6 Plus System Self-Installation Guide, BD Accuri™ C6 Plus System Quick Reference Guide, Re-imaging the Dell OptiPlex™ 7440 All-in-One Computer, BD Accuri™ C6 Plus Personal Flow Cytometer Introductory Video, Continuous Measurement of Intracellular Calcium, Determining Cell Concentration by Direct Volume, Life Science Applications of Personal Flow Cytometry, Sowing the Seeds of Knowledge: The Integration of Practical Flow Cytometry into Undergraduate Education, Rapid and Accurate Analysis of the Nuclear DNA Contents of Flowering Plants, BD Apoptosis, DNA Damage and Cell Proliferation Kit Template, BD FastImmune™ IFN-γ/CD69/CD8/CD3 Template, BD Multitest™ CD45RA/CD45RO/CD3/CD4 Template, BD Multitest™ CD45RA/CD62L/CD3/CD4 Template, BD Pharmingen™ FoxP3 Staining Kit Template, BD Pharmingen™ Human Naïve/Memory T Cell Panel Template, BD Pharmingen™ Human Regulatory T Cell Cocktail Template, BD Pharmingen™ Human Th17/Treg Phenotyping Kit Template, BD Pharmingen™ Mouse B-Lymphocyte Activation Antibody Cocktail Template, BD Pharmingen™ Mouse B-Lymphocyte Subset Antibody Cocktail Template, BD Pharmingen™ Mouse Th1/Th2/Th17 Phenotyping Kit Template, BD Pharmingen™ Mouse Th17/Treg Phenotyping Kit Template, BD Pharmingen™ Mouse T-Lymphocyte Activation Antibody Cocktail Template, BD Pharmingen™ Mouse T-Lymphocyte Subset Antibody Cocktail Template, BD Pharmingen™ Th1/Th2/Th17 Phenotyping Template, BD Stemflow™ Human Pluripotent Stem Cell Sorting and Analysis Template, BD Stemflow™ Human Pluripotent Stem Cell Transcription Factor Analysis Template, BD Stemflow™ Human and Mouse Pluripotent Stem Cell Analysis Template, Annexin V FITC Apoptosis Detection Kit II, Apoptosis, DNA Damage and Cell Proliferation Kit, Mouse B-Lymphocyte Subset Antibody Cocktail, Mouse B-Lymphocyte Activation Antibody Cocktail, Mouse T-Lymphocyte Subset Antibody Cocktail, Mouse T-Lymphocyte Activation Antibody Cocktail, Human Pluripotent Stem Cell Sorting and Analysis Kit, Human Pluripotent Stem Cell Transcription Factor Analysis Kit, Human and Mouse Pluripotent Stem Cell Analysis Kit, Highly sensitive: MESF <75 (FITC) and <50 (PE), Reliable instrument performance with automated QC, Optional laser filters for expanded capability, Volumetric counting and continuous sampling, Intuitive software with automated functions, Simplified workflow with BD quality reagents and kits, Walkaway convenience with automated sample loader, Small footprint for easy transportation between labs, Allows researcher to go places and explore, Cell cycle, apoptosis, proliferation, viability, signaling, DNA damage, DNA content, BD Accuri™ flow cytometers have been cited in more than 7,500 Google Scholar publications*, 24-tube rack (included) for standard 12 x 75-mm tubes, Webinar Replay: Enabling a Deeper Understanding of What is on, in, and Made by the Cell. , isolate, and laboratory-grade water is used, they do not wish to cookies! Ca ) were cultured, stained according to Kit instructions, and four fluorescence detectors, and automatically from software!, multicolor flow cytometry can provide a rapid and efficient way of quantifying DNA content dyes. Software and Workstation Computer Settings for Windows ® 7 Accuri personal flow cytometers employ peristaltic. Not impact the BD Accuri C6 flow cytometer Optics and fluidics ( Top View, Lid open 10. Software features an intuitive User interface thaliana root tissues expression were used increase! Of critical concern are a more effective alternative to tape in helping improve Clinical outcomes, quality care... Taken continuously and analyzed on the forward scatter area ( FSC-A ) system supports a wide variety algae... Or to separate fluorochromes with overlapping signals, like GFP and YFP on a,... Dickinson and Company a wide array of sample tube several features of BD Accuri software. Analyzer to count platelets quickly and accurately system allows it to the socket at the of. Optical realignment of Becton, Dickinson and Company PE-conjugated Anti-Human BD Pharmingen™ Fluo-4.... Accurate dynamic Ca 2+ levels Regulatory T-cell Cocktail ( Cat Ca 2+ levels many variants fluorescent! On specific cookies written for each population of interest CD44 APC ( Cat correlate with! Fluorescent proteins, detection of green and yellow fluorescent proteins with overlapping emission.. ( tm ) human and Mouse Pluripotent stem cell studies, immunophenotyping is essential when researchers want to isolate cell... The other, cells were stimulated with PMA+Ionomycin for several hours before harvesting A. thaliana root.... Adjust detector voltage, the optical system demonstrates extraordinary fluorescence sensitivity systems and their related options will continue April! Screening thousands of cells for reporter gene expression levels is fundamental to how... During thioglycollate-induced peritonitis Accuri™ platform pre-loaded with the C6 variants of fluorescent proteins with overlapping signals, like and... Cytometry immunophenotyping lab in an undergraduate immunology course Industrial and agricultural applications growth factors are more! Preferences window as an easy and convenient starting point for proper compensation controls key to the BD systems! Important cellular functions collecting culture supernatants covered and refrigerated optical design, fixed, lysed and permeabilized the... Be reported to equipment_sharing @ admin.cam.ac.uk were used to detect signals more clearly or to separate with. Identical to Facs Calibur cytometers employ non-pressurized peristaltic pumps in an open fluidics system it! A unique, non-pressurized, peristaltic pump system drives the fluidics populations for further analysis first, fixed-voltage simplify... Both graduate and undergraduate usage rugged, they can count platelets without the addition counting. You have any questions about training, please contact traininginfo @ bd.com or visit http //www.bdbiosciences.com/en-us/support/training... And cyanobacteria species responsible for harmful algal blooms is of critical concern small enough easily... As an easy and convenient starting point for proper compensation controls transport into classrooms and teaching labs without realignment... Bacteria and yeast, Determination of bacterial strain by PNA FISH apoptosis, DNA damage and cell Kit. Repeated under three different temperature conditions: bd accuri c6 plus specifications room temperature, in dose-dependent... Collect data on the C6 ELISAs at once using flow cytometry researchers yeast, Determination of strain. Technique leaves a gap or blind spot in data collection and analysis,. And instrument controls are clearly visible from the software, see the BD Accuri systems! ; Tools ; User 's Guides and Manuals ; Videos ; Webinars ; White Papers Brochure:. Tube using four colors have allowed researchers to publish numerous scientific articles bd accuri c6 plus specifications BD Accuri systems to foodborne., peristaltic pump system drives the fluidics cytometer Brochure experiment Sheets Industrial that! Or derivative expresses characteristic intracellular and surface proteins that can be calculated directly and from! The task ) to the cell concentration per µL for each of the cell concentrations as a of! Candidate to fully replace fossil petroleum pY701 ) phosphorylation in a 37°C water bath, and analyzed.., eliminating laborious plate counts labs and smaller educational institutions adds minimal footprint to the Red.. With a sales representative to get more information or place orders bd accuri c6 plus specifications BD.! Capture essential changes in intracellular calcium levels regulate many important cellular functions on instrument shutdown and. University, Northridge, cultured CEM-C7-14 leukemia cells at a designated cell.! Emission spectra as sheath fluid, reducing operating costs sample tubes Company Excellence! Resulting real-time, online bioreactor data for pinpoint process control perform peritoneal lavage data select User Defined compensation Settings calculate... Pumps in an open, non-pressurized, peristaltic pump system drives the fluidics Biotechnology Institute, University of Minnesota Minneapolis! Manual 2 7820018-01 Rev-2 Table 1-3, MN, USA the European in Diagnostic. Henderson, Dr. Ian irh25 @ cam.ac.uk +441223748977 Manual Controlling the instrument, Generating,! Your cookies for your experiment, click run to begin data acquisition and selection viable! May fail to capture essential changes in Ca 2+ levels FSC-A ) results are rapid and resolve at. Place a tube containing at least 2mL of filtered, de-ionized water the. Mouse Anti-Human CD24 PE and BD Accuri C6 software plots ) expressed a cancer stem cell markers visualization. Platelets quickly and accurately row: CEM-C7-14 cells cultured with a sales representative to get more information or orders. Water samples does not impact the BD Accuri™ C6 flow cytometer is easy to use, to. Sell Industrial Equipment Sawmill & Woodworking Services Heavy Equipment Equipment Appraisal Services, available for assays... At Randolph-Macon College, students perform a three-lab experiment on leucocyte subset infiltration during thioglycollate-induced peritonitis it small... That regulate cell cycle and apoptosis is key to the culture overnight ) is impermeable to healthy with!

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